| Expression pattern: |
DN |
| Associated gene: |
- |
| Associated microRNA: |
miR-1228 |
| Biological function: |
Inhibits proliferation, induces apoptosis, suppresses migration/invasion and EMT in liver cancer cells. |
| Molecular mechanism: |
circ_100395 directly binds miR-1228 and suppresses its expression; miR-1228 mimics reverse circ_100395-induced anti-tumor effects and EMT suppression. |
| Biological pathway or process: |
proliferation (inhibits); apoptosis (promotes); migration (inhibits); invasion (inhibits); EMT (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Survival Analysis; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Luciferase Reporter Assay; Bioinformatics Analysis |
| Clinical significance: |
Overexpression of circ_100395 was negatively associated with tumor differentiation, microvascular invasion, and portal vein tumor thrombosis; higher circ_100395 expression tended to have longer postoperative disease-free survival time. |
| Description: |
circ_100395 is down-regulated in liver cancer and acts as a tumor suppressor. Its overexpression inhibits proliferation, migration/invasion and EMT while promoting apoptosis, at least partly by directly binding and suppressing miR-1228; higher circ_100395 is associated with longer disease-free survival. |
| Confidence score: |
0.7113 |