| Expression pattern: |
UP |
| Associated gene: |
PNISR, TTI1, CNOT10 |
| Associated microRNA: |
- |
| Biological function: |
inhibits KSHV lytic transcription and virus production; promotes cell cycle and cell growth; inhibits apoptosis; induces immune genes |
| Molecular mechanism: |
KSHV induces circ_0001400 co-transcriptionally (likely at back-splicing); circ_0001400 interacts with splicing factor PNISR; circ_0001400 binds RNAs including TTI1 and sustains TTI1 transcript levels, promoting PI3K/AKT/mTOR-associated pro-growth and latency phenotypes |
| Biological pathway or process: |
cell cycle (promotes); apoptosis (inhibits); immune regulation (promotes); PI3K/AKT (promotes); PI3K/AKT/mTOR (promotes); proliferation (promotes); viral replication (inhibits) |
| Detected method: |
Q
S
M
|
| Validation methods: |
RNA-seq; Microarray; RT-qPCR; Bioinformatics Analysis; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; Transduction; EdU Staining; Cell Cycle Assay; Flow Cytometry（Non-apoptosis/cycle）; Annexin V/PI Flow Cytometry; In Vivo Animal Model; Luciferase Reporter Assay; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Northern Blot; FISH / smFISH; ISH (In Situ Hybridization); IHC (Immunohistochemistry); IF (Immunofluorescence); Nuclear-Cytoplasmic Fractionation; Actinomycin D / DRB Stability Assay; circRNA-seq; MTT; CCK8; BrdU; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Tube Formation Assay; TUNEL; H&E Staining; Oil Red O Staining; ELISA; Western Blot; MeRIP / MeRIP-seq; Co-IP; ChIP / ChIP-seq; MSP (Methylation-Specific PCR); Clinical Sample Validation; Cohort Study; Survival Analysis; ROC Analysis; circRIP; CLIP |
| Clinical significance: |
- |
| Description: |
hsa_circ_0001400 (circ_0001400/circRELL1) is induced after KSHV and other herpesvirus infections and biases infected endothelial cells toward latency by suppressing lytic transcription and virion production. Mechanistically, it interacts with the splicing factor PNISR and binds host transcripts including the mTOR-component TTI1, helping sustain TTI1 levels and promoting PI3K/AKT/mTOR-associated pro-growth, anti-apoptotic and immune-gene programs. |
| Confidence score: |
0.9083 |