| Expression pattern: |
UN |
| Associated gene: |
TGIF1, HuR, AGO2, PCNA, Cyclin D1, Bax, Bcl-2, Caspase-3, Caspase-9 |
| Associated microRNA: |
miR-299-3p |
| Biological function: |
promotes GC cell proliferation and viability and suppresses apoptosis; promotes tumor growth in vivo |
| Molecular mechanism: |
CDR1as acts as a cytoplasmic miR-299-3p sponge to relieve suppression of TGIF1; HuR binds CDR1as (via RRM2) and positively regulates CDR1as level and downstream TGIF1, affecting PCNA and Bax |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry) |
| Clinical significance: |
could be used as a new therapeutic target for GC |
| Description: |
In gastric cancer, CDR1as functions as an oncogenic circRNA that enhances proliferation/viability and suppresses apoptosis in vitro and in cell line-derived xenograft tumors. Mechanistically, it acts as a ceRNA sponging miR-299-3p to de-repress TGIF1, while the RBP HuR binds CDR1as and increases its level, strengthening the HuR/CDR1as/miR-299-3p/TGIF1 axis. |
| Confidence score: |
0.7456 |