circRNA basic information
circBase ID: -
Name: hsa_circ_CCNB1
Synonym: circCCNB1
Host Gene: CCNB1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0015459
MONDO name: nasopharyngeal carcinoma
Disease details: nasopharyngeal carcinoma / NPC
Disease DO ID:
 9261
Disease MeSH ID:
 D00007727; D000077274
Disease NCIt ID:
 C3871
Disease ICD11 ID:
 1883313543
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NPC tissues; non-cancerous nasopharyngeal tissues
Cell lines:

HNE2; CNE2
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: NF90, DGCR8, CALU, KIF1B, POLR3G
Associated microRNA: miR-15b-5p, miR-7-1-3p
Biological function: Inhibits vasculogenic mimicry (VM) in nasopharyngeal carcinoma cells.
Molecular mechanism: CircCCNB1 competitively binds NF90 (via NF90 DSRM2 and circCCNB1 loop 1), reduces NF90 binding to pri-miR-15b and pri-miR-7-1, increases DGCR8 binding to these pri-miRNAs, promotes processing/generation of miR-15b-5p and miR-7-1-3p, which repress CALU/KIF1B/POLR3G and decreases VM markers (MMP9, VE-cadherin).
Biological pathway or process:

angiogenesis (inhibits); migration (inhibits); invasion (inhibits); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RT-qPCR; Western Blot; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; Tube Formation Assay; Luciferase Reporter Assay; Bioinformatics Analysis
Clinical significance:

-
Description:

In nasopharyngeal carcinoma, circCCNB1 is down-regulated and suppresses vasculogenic mimicry. Mechanistically, circCCNB1 sequesters the RBP NF90, relieving NF90-mediated inhibition of pri-miR-15b and pri-miR-7-1 processing, thereby increasing miR-15b-5p/miR-7-1-3p and repressing CALU, KIF1B and POLR3G to reduce VM markers.
Confidence score:

0.6412
Other information
Title:

CircCCNB1 inhibits vasculogenic mimicry by sequestering NF90 to promote miR-15b-5p and miR-7-1-3p processing in nasopharyngeal carcinoma.

Journal: Molecular oncology
Published: 2025
PubMed ID: 39964093
Study type:

biological research
Data availability: -
Code availability: -