| Expression pattern: |
UP |
| Associated gene: |
integrin-beta1 (ITGB1) |
| Associated microRNA: |
miR-153-3p |
| Biological function: |
Promotes NSCLC cell viability and proliferation, promotes invasion, and inhibits apoptosis; propofol restrains tumor growth partly by downregulating circ_0000735. |
| Molecular mechanism: |
circ_0000735 acts as a miR-153-3p sponge/decoy to regulate ITGB1 expression; propofol downregulates circ_0000735, increasing miR-153-3p and decreasing ITGB1. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
divergent primers PCR; RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Bioinformatics Analysis; Transfection; MTT; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Clinical Sample Validation |
| Clinical significance: |
- |
| Description: |
circ_0000735 is up-regulated in NSCLC and promotes malignant phenotypes (viability/proliferation/invasion) while inhibiting apoptosis. It mainly functions as a ceRNA by sponging miR-153-3p to increase ITGB1 expression, and propofol suppresses NSCLC growth partly by downregulating circ_0000735 and thereby reducing ITGB1 via the circ_0000735/miR-153-3p axis. |
| Confidence score: |
0.8174 |