| Expression pattern: |
UP |
| Associated gene: |
GNG5 |
| Associated microRNA: |
miR-656-3p |
| Biological function: |
promotes cell growth, migration, invasion and glycolysis; inhibits apoptosis |
| Molecular mechanism: |
ceRNA mechanism: circ-PAPPA sponges miR-656-3p to upregulate GNG5, enhancing glycolysis and malignant phenotypes in colon cancer cells |
| Biological pathway or process: |
glycolysis (promotes); proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Western Blot |
| Clinical significance: |
High expression of circ-PAPPA was associated with TNM stage, lymphatic metastasis, tumor size, distant metastasis, and predicted poor prognosis / lower 5-year survival rates in CC patients |
| Description: |
circ-PAPPA (hsa_circ_0088233) is up-regulated in colon cancer and promotes malignant behaviors and aerobic glycolysis. Mechanistically, it sponges miR-656-3p to relieve repression of GNG5 (miR-656-3p/GNG5 axis), and higher circ-PAPPA is associated with worse survival. |
| Confidence score: |
0.713 |