| Expression pattern: |
UP |
| Associated gene: |
TET2, MMP2, MMP9 |
| Associated microRNA: |
miR-143 |
| Biological function: |
promotes proliferation, migration, invasion, tumor growth and metastasis in gastric cancer |
| Molecular mechanism: |
ceRNA mechanism: hsa_circRNA_101996 sponges miR-143 to upregulate TET2, which regulates MMP2/MMP9 via DNA demethylation |
| Biological pathway or process: |
ceRNA regulation (promotes); invasion (promotes); migration (promotes); proliferation (promotes); metastasis (promotes); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
Bioinformatics Analysis; RT-qPCR; Transwell Assay; Western Blot; Luciferase Reporter Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Transfection; Clinical Sample Validation; Survival Analysis; In Vivo Animal Model |
| Clinical significance: |
High hsa_circRNA_101996 expression correlates with tumor size, lymphatic metastasis, distant metastasis, and lower three-year survival rate; plasma level decreases after surgery but remains higher than healthy controls |
| Description: |
hsa_circRNA_101996 is up-regulated in gastric cancer tissues/cell lines and mainly localizes to the cytoplasm. It promotes GC proliferation, migration, invasion and in vivo tumor growth by sponging miR-143, thereby de-repressing TET2 and increasing MMP2/MMP9 expression via a DNA demethylation-related mechanism; high expression indicates worse survival. |
| Confidence score: |
0.7444 |