| Expression pattern: |
UP |
| Associated gene: |
PDK2, AGO2 |
| Associated microRNA: |
miR-330-5p |
| Biological function: |
Facilitates mitochondrial metabolism and supports HuMSCs function under hypoxia; contributes to endometrial repair by decreasing fibrosis area and increasing gland number in IUA model. |
| Molecular mechanism: |
Acts as a miRNA sponge/ceRNA by binding miR-330-5p, thereby relieving miR-330-5p-mediated suppression of PDK2 and stabilizing mitochondrial metabolism; functional rescue supports circPTP4A2-miR-330-5p-PDK2 signaling in endometrial repair. |
| Biological pathway or process: |
mitochondrial function (promotes); oxidative phosphorylation (promotes); fibrosis (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Western Blot; In Vivo Animal Model; H&E Staining |
| Clinical significance: |
- |
| Description: |
circPTP4A2 is upregulated in HuMSCs cultured on SF-SIS scaffolds and in transplanted endometrial tissues in an IUA mouse model. It mainly localizes to the cytoplasm and promotes HuMSC mitochondrial metabolism under hypoxia by sponging miR-330-5p to regulate PDK2, thereby improving endometrial repair (more glands and less fibrosis). |
| Confidence score: |
0.7385 |