| Expression pattern: |
UP |
| Associated gene: |
SRSF1, TXNIP |
| Associated microRNA: |
- |
| Biological function: |
promotes oxidative stress, inflammation, autophagy-dependent ferroptosis and apoptosis in MIRI models |
| Molecular mechanism: |
circHIPK2 recruits the RBP SRSF1 to TXNIP promoter/targeting and increases TXNIP mRNA stability |
| Biological pathway or process: |
ferroptosis (promotes); autophagy (promotes); apoptosis (promotes); inflammation (promotes); oxidative phosphorylation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); ChIP / ChIP-seq; Actinomycin D / DRB Stability Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; ELISA; Western Blot; In Vivo Animal Model; H&E Staining |
| Clinical significance: |
- |
| Description: |
circHIPK2 is up-regulated in H/R-treated human cardiomyocytes and in I/R mouse heart tissue. It promotes oxidative stress, inflammation, autophagy-dependent ferroptosis and apoptosis by recruiting SRSF1 to enhance TXNIP transcriptional recruitment and stabilize TXNIP mRNA, and circHIPK2 knockdown alleviates MIRI phenotypes in vitro and in vivo. |
| Confidence score: |
0.7014 |