| Expression pattern: |
UP |
| Associated gene: |
SLC7A11, EIF4A3, p-AKT |
| Associated microRNA: |
let-7a-5p |
| Biological function: |
promotes proliferation and migration of breast cancer cells; knockdown contributes to disulfidptosis; promotes tumor growth in vivo |
| Molecular mechanism: |
circ_0022382 sponges let-7a-5p to activate PI3K/AKT/mTOR signaling and increase SLC7A11; EIF4A3 promotes circ_0022382 production (RBP-related regulation) |
| Biological pathway or process: |
PI3K/AKT/mTOR (promotes); proliferation (promotes); migration (promotes); disulfidptosis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Bioinformatics Analysis; Microarray; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Transwell Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); IF (Immunofluorescence); Colony Formation Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot |
| Clinical significance: |
may be effective targets for diagnosis or targeted therapy of breast cancer |
| Description: |
circ_0022382 is up-regulated in breast cancer tissues and cell lines and promotes proliferation and migration. Mechanistically, it sponges let-7a-5p to activate PI3K/AKT/mTOR signaling and increase SLC7A11, while its knockdown induces features consistent with disulfidptosis and suppresses tumor growth in a xenograft model; EIF4A3 promotes circ_0022382 biogenesis. |
| Confidence score: |
0.7761 |