| Expression pattern: |
UP |
| Associated gene: |
SFPQ, hnRNPA2B1, FBXW11 |
| Associated microRNA: |
- |
| Biological function: |
Promotes GC cell proliferation, migration, cell cycle G1/S transition, tumor growth and metastasis; suppresses antitumour activity of T cells. |
| Molecular mechanism: |
Exosomal circMAN1A2 binds SFPQ (RRM1 domain), competes with FBXW11 for SFPQ binding, prevents FBXW11-mediated K48-linked ubiquitination and proteasomal degradation of SFPQ, stabilizing SFPQ; packaged into exosomes via hnRNPA2B1; inhibits TCR signalling activation in T cells. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); metastasis (promotes); cell cycle (promotes); immune regulation (inhibits); other pathway/process (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; RT-qPCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; divergent primers PCR; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Clinical Sample Validation; Survival Analysis; ROC Analysis; Transfection; Colony Formation Assay; CCK8; EdU Staining; Wound Healing Assay; Transwell Assay; Cell Cycle Assay; Flow Cytometry（Non-apoptosis/cycle）; ELISA; Annexin V/PI Flow Cytometry; TUNEL; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; RIP (RNA Immunoprecipitation); RNA Pull-Down; IF (Immunofluorescence); Co-IP; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
High circMAN1A2 expression correlates with lymph node metastasis and TNM stage, predicts worse prognosis, and plasma exosomal circMAN1A2 (especially combined with CEA) has diagnostic value for GC. |
| Description: |
circMAN1A2 (hsa_circ_0000118) is upregulated and enriched in GC-derived exosomes and is associated with poor prognosis and diagnostic potential in plasma exosomes. Functionally, exosomal circMAN1A2 promotes GC proliferation/migration and metastasis and suppresses T-cell antitumour effector functions. Mechanistically, it is packaged into exosomes via hnRNPA2B1 and binds SFPQ to block FBXW11-mediated K48-linked ubiquitination and proteasomal degradation of SFPQ, stabilizing SFPQ and inhibiting TCR signalling in T cells. |
| Confidence score: |
0.8977 |