circRNA basic information
circBase ID: -
Name: hsa_circ_RPPH1
Synonym: circRPPH1
Host Gene: RPPH1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0021042
MONDO name: glioma
Disease details: glioma
Disease DO ID:
 -
Disease MeSH ID:
 D005910
Disease NCIt ID:
 C3059
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

glioma tissues; adjacent tissues
Cell lines:

U87; T98G; HS683; U373; LN-319; SW1783; U251; HEB
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: c-FOS
Associated microRNA: -
Biological function: Promotes glioma cell stemness, sphere-formation ability, ALDH activity, stemness marker expression, and tumor-initiating ability in vivo.
Molecular mechanism: c-FOS transcriptionally activates circRPPH1 expression by directly binding to the circRPPH1 promoter; circRPPH1 promotes glioma stemness dependent on c-FOS-mediated activation.
Biological pathway or process:

stemness (promotes); proliferation (promotes); other pathway/process (promotes)
Detected method:
Q
M
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Transfection; Western Blot; Luciferase Reporter Assay; ChIP / ChIP-seq; In Vivo Animal Model; Bioinformatics Analysis
Clinical significance:

-
Description:

circRPPH1 is up-regulated in glioma cells, glioma cell spheres, and glioma tissues based on RT-qPCR and online dataset analysis. Functionally, circRPPH1 promotes glioma stemness, including CSC marker expression, sphere formation, ALDH activity, and tumor-initiating capacity in vivo. Mechanistically, c-FOS binds the circRPPH1 promoter and transcriptionally activates circRPPH1 expression, forming a c-FOS/circRPPH1 regulatory axis.
Confidence score:

0.7281
Other information
Title:

C-Fos-activated circRPPH1 contributes to glioma stemness.

Journal: Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico
Published: 2023
PubMed ID: 36454517
Study type:

biological research
Data availability: All data generated or analyzed during this study are included in this published article.
Code availability: -