| Expression pattern: |
UP |
| Associated gene: |
CDK6 |
| Associated microRNA: |
miR-1299 |
| Biological function: |
Promotes breast cancer cell proliferation, survival, cell cycle progression in G2/M phase, migration and invasion. |
| Molecular mechanism: |
hsa_circ_0136666 acts as a competing endogenous RNA by sponging miR-1299, thereby positively modulating CDK6 expression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Cell Cycle Assay; Transwell Assay; Western Blot |
| Clinical significance: |
hsa_circ_0136666 is provided as a significant biomarker for breast cancer treatment. |
| Description: |
hsa_circ_0136666 is up-regulated in breast cancer cells and tissues. It promotes breast cancer progression by enhancing proliferation, cell-cycle progression, migration and invasion, at least partly through sponging miR-1299 and positively regulating CDK6 expression. The authors propose the hsa_circ_0136666/miR-1299/CDK6 axis as a potential biomarker and therapeutic target for breast cancer. |
| Confidence score: |
0.7045 |