| Expression pattern: |
UP |
| Associated gene: |
- |
| Associated microRNA: |
miR-29c-3p |
| Biological function: |
hsa_circ_0001564 promotes osteosarcoma tumorigenicity by enhancing proliferation and colony formation, facilitating cell cycle progression, and inhibiting apoptosis; knockdown suppresses proliferation, induces G0/G1 cell cycle arrest, and promotes apoptosis in HOS and MG-63 cells. |
| Molecular mechanism: |
hsa_circ_0001564 acts as a miR-29c-3p sponge and exerts oncogenic effects in osteosarcoma through a competing endogenous RNA mechanism. |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Microarray; Clinical Sample Validation; Luciferase Reporter Assay; Bioinformatics Analysis; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay |
| Clinical significance: |
hsa_circ_0001564 could act as a potential biomarker and therapeutic target for osteosarcoma. |
| Description: |
hsa_circ_0001564 is up-regulated in osteosarcoma tissues and cell lines and functions as an oncogenic circRNA. Its knockdown suppresses proliferation and colony formation, induces G0/G1 cell cycle arrest, and promotes apoptosis, at least partly by sponging miR-29c-3p. The study suggests hsa_circ_0001564 may serve as a potential biomarker and therapeutic target for osteosarcoma. |
| Confidence score: |
0.7404 |