| Expression pattern: |
UP |
| Associated gene: |
- |
| Associated microRNA: |
- |
| Biological function: |
Circ-IGF1R promotes HCC cell proliferation and inhibits apoptosis and cell cycle arrest; knockdown suppresses proliferation, promotes apoptosis, and induces G0/G1 cell cycle arrest. |
| Molecular mechanism: |
Circ-IGF1R exerts oncogenic, pro-proliferative and anti-apoptotic effects at least partly through activation of the PI3K/AKT pathway, with downstream changes in Bcl-2, Bax, caspase-3 and cleaved caspase-3. |
| Biological pathway or process: |
PI3K/AKT (promotes); proliferation (promotes); apoptosis (inhibits); cell cycle (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
Sanger Sequencing; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
High circ-IGF1R expression in HCC was associated with tumour size and may be a potential target for HCC prevention and treatment. |
| Description: |
This study identified circ-IGF1R (hsa_circ_0005035) as an IGF1R-derived circRNA upregulated in HCC tissues and HCC cell lines. High circ-IGF1R expression was associated with larger tumor size, and circ-IGF1R knockdown inhibited HCC cell proliferation while inducing apoptosis and G0/G1 cell cycle arrest. Mechanistically, circ-IGF1R appears to promote HCC progression at least partly by activating the PI3K/AKT pathway and modulating apoptosis-related proteins including Bcl-2, Bax and caspase-3. |
| Confidence score: |
0.6536 |