| Expression pattern: |
UP |
| Associated gene: |
AKR1C3, AGO2 |
| Associated microRNA: |
miR-379-5p |
| Biological function: |
promotes proliferation, migration, and invasion; promotes tumor growth in vivo |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-379-5p to up-regulate AKR1C3 expression (ceRNA regulation). |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); PI3K/AKT (other); ceRNA regulation (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; Clinical Sample Validation; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RNase R Treatment; Actinomycin D / DRB Stability Assay; Sanger Sequencing; RNA-seq; Western Blot; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
Elevated circPTK2 expression was positively correlated with advanced tumor T stage and vascular invasion; circPTK2 expression was closely related to tumor size, T stage, hepatitis B virus infection, and microvascular invasion. |
| Description: |
hsa_circ_0008305 (circPTK2) is up-regulated in HCC and mainly localized in the cytoplasm. It promotes HCC cell proliferation, migration, invasion, and xenograft tumor growth by sponging miR-379-5p to increase AKR1C3 expression (circPTK2/miR-379-5p/AKR1C3 ceRNA axis). |
| Confidence score: |
0.8781 |