| Expression pattern: |
UN |
| Associated gene: |
anti-PD-1 scFv |
| Associated microRNA: |
miR-25 |
| Biological function: |
Inhibits HCC cell proliferation, invasion/migration, EMT and angiogenesis; enhances antitumor immunity via anti-PD-1 scFv expression. |
| Molecular mechanism: |
Acts as a miR-25 sponge and expresses anti-PD-1 scFv to block PD-1/PD-L1 signaling and enhance immune response. |
| Biological pathway or process: |
proliferation (inhibits); invasion (inhibits); migration (inhibits); EMT (inhibits); angiogenesis (inhibits); immune regulation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Sanger Sequencing; RT-qPCR; Transfection; Western Blot; Flow Cytometry（Non-apoptosis/cycle）; Transwell Assay; Wound Healing Assay; EdU Staining; In Vivo Animal Model; IHC (Immunohistochemistry); IF (Immunofluorescence); ELISA; H&E Staining; Bioinformatics Analysis; Survival Analysis |
| Clinical significance: |
- |
| Description: |
This engineered artificial circRNA (circSC25-alphaPD1) functions as a miR-25 sponge and simultaneously expresses anti-PD-1 scFv. It suppresses HCC malignant phenotypes (proliferation, invasion/migration, EMT, angiogenesis) and enhances antitumor immunity, leading to inhibited tumor growth and prolonged survival in mouse models. |
| Confidence score: |
0.7554 |