| Expression pattern: |
UP |
| Associated gene: |
IGF2, Ago2 |
| Associated microRNA: |
miR-98-5p |
| Biological function: |
promotes paclitaxel resistance (chemoresistance) and cell growth in PTX-resistant NSCLC cells; its knockdown enhances PTX sensitivity |
| Molecular mechanism: |
acts as a miRNA sponge for miR-98-5p to increase IGF2 expression, thereby promoting PTX resistance |
| Biological pathway or process: |
chemoresistance (promotes); proliferation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Transfection; MTT; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
hsa_circ_0003489 levels are associated with tumor size, TNM stages, distant metastasis or recurrence, and lymph nodes metastasis in NSCLC patients |
| Description: |
hsa_circ_0003489 is up-regulated in paclitaxel-resistant NSCLC tissues and resistant cell models. Silencing hsa_circ_0003489 reduces PTX IC50 and inhibits cell growth by sponging miR-98-5p and downregulating IGF2, indicating a ceRNA axis that promotes chemoresistance. |
| Confidence score: |
0.7688 |