| Expression pattern: |
UP |
| Associated gene: |
ELAVL1, ELANE |
| Associated microRNA: |
- |
| Biological function: |
promotes neutrophil extracellular trap (NET) formation and increases elastase expression |
| Molecular mechanism: |
circIRAK3 binds ELAVL1 to enhance ELANE mRNA stability, increasing elastase protein expression and thereby promoting NET formation |
| Biological pathway or process: |
mRNA stability (promotes); immune regulation (other); inflammation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; Bioinformatics Analysis; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; Western Blot; IF (Immunofluorescence); Flow Cytometry（Non-apoptosis/cycle）; ELISA; ROC Analysis; Clinical Sample Validation |
| Clinical significance: |
circIRAK3 exhibited favorable biomarker potential to distinguish ICU septic patients from non-septic critically ill patients (AUC=0.840) and its expression positively correlated with plasma NET levels |
| Description: |
circIRAK3 is up-regulated in peripheral blood neutrophils from sepsis patients and correlates with plasma NET levels. Functionally, it promotes NET formation by binding ELAVL1 and enhancing ELANE mRNA stability, increasing elastase expression. |
| Confidence score: |
0.8701 |