| Expression pattern: |
UP |
| Associated gene: |
PDSS1, Akt, ERK1/2, JNK, p38, mTORC1, mTORC2, p70S6K, Bax, cleaved caspase-3, Ki-67, PCNA |
| Associated microRNA: |
hsa-miR-4436a, hsa-miR-502-3p |
| Biological function: |
promotes OS cell proliferation and invasion, and inhibits apoptosis; promotes tumorigenesis in vivo |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-502-3p and miR-4436a; modulates prosurvival signaling (Akt/ERK/JNK/p38/mTOR/p70S6K phosphorylation) and pro-apoptotic proteins (Bax, cleaved caspase-3). |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); apoptosis (inhibits); PI3K/AKT (promotes); ERK (promotes); MAPK (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; In Vivo Animal Model; Survival Analysis |
| Clinical significance: |
Patients with high circPDSS1 expression were associated with poorer progression-free survival (PFS) and overall survival (OS). |
| Description: |
circPDSS1 is up-regulated in osteosarcoma tissues and cell lines and is associated with worse patient survival. Functionally, circPDSS1 promotes proliferation, clonogenic growth, invasion and in vivo tumorigenesis while inhibiting apoptosis. Mechanistically, it mainly localizes to the cytoplasm and sponges miR-502-3p and miR-4436a, influencing survival signaling (Akt/MAPK-related phosphorylation changes). |
| Confidence score: |
0.7592 |