| Expression pattern: |
UP |
| Associated gene: |
PGAM1, PABPC1, MKRN3, c-Myc |
| Associated microRNA: |
- |
| Biological function: |
Promotes glycolysis and hepatocellular carcinoma cell proliferation/tumor growth via increasing PGAM1 expression. |
| Molecular mechanism: |
circDDX21 binds PABPC1, disrupts MKRN3-PABPC1 interaction, inhibits MKRN3-mediated PABPC1 ubiquitination, enhances PABPC1 binding to PGAM1 mRNA and stabilizes PGAM1 mRNA. |
| Biological pathway or process: |
glycolysis (promotes); proliferation (promotes); ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; Sanger Sequencing; Back-Splice Junction PCR / divergent primers PCR; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; Western Blot; Colony Formation Assay; In Vivo Animal Model; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
circDDX21 is highly expressed in clinical hepatocellular carcinoma tissues compared to normal tissues, and circDDX21 expression is positively correlated with PGAM1 expression. |
| Description: |
In hepatocellular carcinoma, circDDX21 is upregulated and promotes glycolysis and tumor growth. It binds the RBP PABPC1, reduces MKRN3-mediated PABPC1 ubiquitination, enhances PABPC1 binding to PGAM1 mRNA, and stabilizes PGAM1 mRNA to increase PGAM1 expression. |
| Confidence score: |
0.8547 |