| Expression pattern: |
UP |
| Associated gene: |
PFN2, PAX5, AGO2, TGF-beta1, Smad2, Smad3 |
| Associated microRNA: |
miR-183-5p |
| Biological function: |
promotes proliferation, migration, invasion, EMT, and liver metastasis of CRC cells |
| Molecular mechanism: |
PAX5-induced upregulation of circ0020134; circ0020134 sponges miR-183-5p to upregulate PFN2 and activate the TGF-beta/Smad pathway, promoting EMT and metastasis |
| Biological pathway or process: |
TGF-beta/SMAD (promotes); EMT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Survival Analysis; Bioinformatics Analysis; Clinical Sample Validation; Cohort Study |
| Clinical significance: |
High circ0020134 levels were associated with poor prognosis (worse 5-year OS) in CRC patients. |
| Description: |
hsa_circ_0020134 (circ0020134), derived from CACUL1, is up-regulated in CRC liver metastases and is associated with worse overall survival. It promotes CRC proliferation and metastasis by sponging miR-183-5p to increase PFN2 expression, activating TGF-beta/Smad signaling and EMT; its expression is transcriptionally induced by PAX5. |
| Confidence score: |
0.8941 |