| Expression pattern: |
UP |
| Associated gene: |
hnRNPU, VEGF-C, H3K27ac |
| Associated microRNA: |
- |
| Biological function: |
Promotes lymphangiogenesis and lymph node metastasis in LUAD. |
| Molecular mechanism: |
circUBE2G1 binds hnRNPU and recruits it to the VEGF-C promoter to enhance H3K27ac, activating VEGF-C transcription and increasing VEGF-C secretion. |
| Biological pathway or process: |
angiogenesis (promotes); metastasis (promotes); migration (promotes); proliferation (promotes); ceRNA regulation (other) |
| Detected method: |
Q
H
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Clinical Sample Validation; FISH / smFISH; divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Transfection; RNA Pull-Down; Western Blot; RIP (RNA Immunoprecipitation); ChIP / ChIP-seq; Luciferase Reporter Assay; Tube Formation Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); ELISA; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circUBE2G1 expression is associated with LN metastasis and poorer OS and DFS in patients with LUAD. |
| Description: |
circUBE2G1 (hsa_circ_0041555) is upregulated in LUAD and is associated with lymph node metastasis and poor survival. It predominantly localizes to the nucleus and promotes lymphangiogenesis and LN metastasis by binding hnRNPU and recruiting it to the VEGF-C promoter to increase H3K27ac and activate VEGF-C transcription/secretion. |
| Confidence score: |
0.897 |