| Expression pattern: |
UP |
| Associated gene: |
UCP2, Ago2 |
| Associated microRNA: |
miR-149 |
| Biological function: |
promotes proliferation, invasion and metastasis of NSCLC cells |
| Molecular mechanism: |
ceRNA mechanism: circUCP2 sponges miR-149 to upregulate UCP2; involves Ago2-linked RISC |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining |
| Clinical significance: |
circUCP2 may therefore be a novel diagnostic biomarker for the progression of NSCLC |
| Description: |
circUCP2 (hsa_circ_0023525) is up-regulated in NSCLC tissues and cells and promotes proliferation, invasion, and metastasis. Mechanistically, it mainly localizes to the cytoplasm and acts as a ceRNA that sponges miR-149, thereby increasing UCP2 expression (circUCP2/miR-149/UCP2 axis). |
| Confidence score: |
0.8397 |