| Expression pattern: |
UP |
| Associated gene: |
eIF4A3, GPX2 |
| Associated microRNA: |
- |
| Biological function: |
promotes M1 macrophage polarization and macrophage activation; promotes macrophage-endothelial crosstalk and endothelial angiogenic behaviors; exacerbates retinal inflammation and retinal vascular dysfunction in diabetes |
| Molecular mechanism: |
cSPECC1 recruits the RBP eIF4A3 to enhance GPX2 mRNA stability, altering arachidonic acid metabolism and increasing 12-HETE production |
| Biological pathway or process: |
arachidonic acid metabolism (promotes); lipid metabolism (promotes); inflammation (promotes); angiogenesis (promotes); proliferation (promotes); migration (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Clinical Sample Validation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; ELISA; Western Blot; IF (Immunofluorescence); Flow Cytometry（Non-apoptosis/cycle）; EdU Staining; Transwell Assay; Tube Formation Assay; In Vivo Animal Model |
| Clinical significance: |
- |
| Description: |
cSPECC1 is up-regulated in diabetic retinas and high-glucose-stressed macrophages and promotes M1-like macrophage activation and pro-angiogenic macrophage-endothelial crosstalk. Mechanistically, cSPECC1 binds/recruits eIF4A3 to enhance GPX2 mRNA stability, reshaping arachidonic acid metabolism and increasing 12-HETE, thereby exacerbating retinal inflammation and vascular dysfunction. |
| Confidence score: |
0.8219 |