| Expression pattern: |
UP |
| Associated gene: |
ATG14, AGO2 |
| Associated microRNA: |
miR-424-5p |
| Biological function: |
Promotes HCC cell proliferation and autophagy, facilitates cell cycle progression, and suppresses apoptosis. |
| Molecular mechanism: |
circCBFB acts as a ceRNA/sponge for miR-424-5p, thereby upregulating ATG14 and triggering autophagy to promote HCC progression. |
| Biological pathway or process: |
proliferation (promotes); autophagy (promotes); apoptosis (inhibits); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; MTT; CCK8; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circCBFB expression is associated with poor prognosis and decreased 5-year survival rate in HCC patients. |
| Description: |
circCBFB is up-regulated in HCC tissues and cell lines and its high expression is associated with poor 5-year survival. Functionally, circCBFB promotes HCC cell proliferation, cell cycle progression, and autophagy while inhibiting apoptosis. Mechanistically, it acts as a cytoplasmic ceRNA that sponges miR-424-5p to upregulate ATG14. |
| Confidence score: |
0.7855 |