| Expression pattern: |
UP |
| Associated gene: |
SOX4, MEX3A, PI3K/AKT pathway |
| Associated microRNA: |
miR-4428 |
| Biological function: |
circGRAMD1B promotes migration, invasion, and epithelial-mesenchymal transition of LUAD cells. |
| Molecular mechanism: |
circGRAMD1B acts as a ceRNA by sponging miR-4428 to upregulate SOX4; SOX4 transcriptionally activates MEX3A, thereby activating the PI3K/AKT pathway. |
| Biological pathway or process: |
PI3K/AKT (promotes); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; ChIP / ChIP-seq; Transfection; Transwell Assay; Wound Healing Assay; IF (Immunofluorescence); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
The authors suggest circGRAMD1B might offer a novel biomarker for LUAD clinical therapies, but no clinical samples were involved. |
| Description: |
circGRAMD1B is up-regulated in LUAD cells and functions as an oncogenic circRNA that promotes LUAD cell migration, invasion, and EMT. Mechanistically, cytoplasmic circGRAMD1B sponges miR-4428 to increase SOX4 expression, leading to SOX4-mediated transcriptional activation of MEX3A and activation of the PI3K/AKT pathway. |
| Confidence score: |
0.7986 |