| Expression pattern: |
UP |
| Associated gene: |
NCBP2, FXR1, NACA, ARPC1B |
| Associated microRNA: |
- |
| Biological function: |
promotes cell growth, migration, invasion/metastasis and tumor growth in osteosarcoma |
| Molecular mechanism: |
circZMYM2 binds FXR1 as an RBP sponge and regulates post-transcriptional translation of NACA and ARPC1B; its biogenesis is regulated by NCBP2 binding to flanking intronic sequences |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); cell cycle (promotes); apoptosis (inhibits); other pathway/process (other) |
| Detected method: |
Q
S
M
|
| Validation methods: |
RNA-seq; Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; In Vivo Animal Model; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
potential diagnostic and therapeutic biomarker for OS patients |
| Description: |
circZMYM2 (hsa_circ_0029634), derived from ZMYM2, is up-regulated in osteosarcoma tissues and mainly localizes to the cytoplasm. It promotes osteosarcoma proliferation and metastasis by competitively binding the RBP FXR1, thereby modulating translation of NACA and ARPC1B; its biogenesis is promoted by NCBP2 binding to flanking intronic regions. |
| Confidence score: |
0.8397 |