| Expression pattern: |
UP |
| Associated gene: |
KPNA2, Ago2 |
| Associated microRNA: |
miR-495-3p |
| Biological function: |
promotes NSCLC tumorigenesis and progression by promoting proliferation, migration/metastasis and oncogenesis |
| Molecular mechanism: |
ceRNA sponge of miR-495-3p to modulate KPNA2 expression |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); metastasis (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; Microarray; Bioinformatics Analysis; Nuclear-Cytoplasmic Fractionation; divergent primers PCR; RNase R Treatment; CCK8; Colony Formation Assay; Transwell Assay; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; In Vivo Animal Model; Clinical Sample Validation; Survival Analysis; Transfection |
| Clinical significance: |
Upregulation of circ_0022383 was associated with poor prognosis in NSCLC patients; upregulation was closely associated with tumor stage III and IV and lymph node metastasis |
| Description: |
circ_0022383 is up-regulated in NSCLC tissues and cell lines and is associated with advanced stage, lymph node metastasis, and poor prognosis. Functionally, it promotes proliferation and migration/metastasis, and in vivo tumor growth. Mechanistically, it mainly localizes to the cytoplasm and acts as a ceRNA sponge for miR-495-3p to regulate KPNA2. |
| Confidence score: |
0.8632 |