| Expression pattern: |
UP |
| Associated gene: |
integrin beta1, Tks5 |
| Associated microRNA: |
- |
| Biological function: |
promotes tumor progression, migration, invasion, metastasis, and invadopodia formation via exosomal transfer to recipient tumor cells |
| Molecular mechanism: |
Exosome-mediated horizontal transfer of circSKA3 to recipient tumor cells; circSKA3 forms complexes with integrin beta1 and Tks5 in exosomes/invadopodia to potentiate invasion and migration |
| Biological pathway or process: |
migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RT-qPCR; FISH / smFISH; ISH (In Situ Hybridization); Clinical Sample Validation; Transfection; Transwell Assay; Wound Healing Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Western Blot; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; H&E Staining |
| Clinical significance: |
Higher circSKA3 levels in ascites exosomes of breast cancer patients compared with non-cancer/healthy individuals |
| Description: |
circSKA3 is up-regulated in ascites exosomes from breast cancer patients and can be horizontally transferred between tumor cells via exosomes. Exosomal circSKA3 complexes with integrin beta1 and Tks5, enhancing invadopodia formation and promoting migration/invasion and tumor progression in vitro and in vivo. |
| Confidence score: |
0.774 |