| Expression pattern: |
UP |
| Associated gene: |
Sirt1 |
| Associated microRNA: |
miR-212-3p |
| Biological function: |
Promotes inflammasome activity, macrophage inflammatory polarization/activation, and inflammatory lung tissue damage; circVAPA knockdown reduces pyroptosis-related proteins and inflammatory cytokines/ROS. |
| Molecular mechanism: |
circVAPA modulates the miR-212-3p/Sirt1 axis (ceRNA-like regulation validated by luciferase reporter), affecting inflammasome/pyroptosis signaling in macrophages. |
| Biological pathway or process: |
inflammation (promotes); pyroptosis (promotes); immune regulation (promotes); apoptosis (promotes); proliferation (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; ELISA; Western Blot; Luciferase Reporter Assay; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; H&E Staining; Bioinformatics Analysis |
| Clinical significance: |
High circVAPA expression in alveolar macrophages from ARDS patients is associated with inflammation/oxidative stress, suggesting potential therapeutic targeting. |
| Description: |
circVAPA is upregulated in alveolar macrophages from ARDS patients and in LPS-induced mouse macrophages. circVAPA promotes macrophage inflammatory polarization and inflammasome/pyroptosis activation by regulating the miR-212-3p/Sirt1 axis, and its knockdown alleviates lung inflammation and tissue injury in an LPS-induced mouse ARDS model. |
| Confidence score: |
0.6545 |