| Expression pattern: |
UP |
| Associated gene: |
PLAG1 like zinc finger 2 (PLAGL2), PLAGL2 |
| Associated microRNA: |
miR-1270 |
| Biological function: |
promotes HCC cell proliferation, migration, invasion and EMT process |
| Molecular mechanism: |
circRNA-103809 functions as a ceRNA by sponging miR-1270 and up-regulating PLAGL2 |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Western Blot; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Bioinformatics Analysis |
| Clinical significance: |
potential diagnostic biomarker and therapeutic target for HCC |
| Description: |
circRNA-103809 is up-regulated in HCC cell lines and acts as an oncogenic circRNA. It promotes proliferation, migration, invasion and EMT by sponging miR-1270 and increasing PLAGL2 expression, forming a circRNA-103809/miR-1270/PLAGL2 ceRNA axis with potential biomarker and therapeutic relevance. |
| Confidence score: |
0.7006 |