| Expression pattern: |
DN |
| Associated gene: |
DKK1, Ago2 |
| Associated microRNA: |
miR-6807-3p |
| Biological function: |
inhibits cell proliferation and stemness; promotes apoptosis; inhibits tumor growth |
| Molecular mechanism: |
circ_0018414 sponges miR-6807-3p to protect DKK1 mRNA from silencing, upregulating DKK1 and inactivating the Wnt/beta-catenin pathway |
| Biological pathway or process: |
Wnt/beta-catenin (inhibits); proliferation (inhibits); apoptosis (promotes); stemness (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; circRIP; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; TUNEL; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
low expression of circ_0018414 predicted unfavorable prognosis of LUAD patients |
| Description: |
hsa_circ_0018414 (circ_0018414), derived from DKK1, is down-regulated in LUAD and functions as a tumor suppressor. It mainly localizes to the cytoplasm and acts as a ceRNA by sponging miR-6807-3p to relieve repression of DKK1, thereby inhibiting Wnt/beta-catenin signaling and reducing proliferation/stemness while promoting apoptosis; low circ_0018414 is associated with worse overall survival. |
| Confidence score: |
0.8748 |