circRNA basic information
circBase ID: hsa_circ_0067934
Name: hsa_circ_PRKCI
Synonym: circ_0067934
Host Gene: PRKCI
Genomic location(hg19): chr3:170013698-170015181:+
Genomic location(hg38): chr3:170295910-170297393:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005075
MONDO name: thyroid gland papillary carcinoma
Disease details: papillary thyroid carcinoma / PTC
Disease DO ID:
 3969
Disease MeSH ID:
 -
Disease NCIt ID:
 C4035
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

papillary thyroid carcinoma specimens; paired normal tissues; adjacent normal tissues; tumor xenografts
Cell lines:

HEK293; HEK293T; Nthy-ori3-1; TPC-1; SW579; FTC133; FTC113; MDA-T32; MDA-T120; XTC-1; KAT-5
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: HMGB1, PI3K/Akt, MAPK
Associated microRNA: miR-1301-3p
Biological function: Promotes PTC cell growth, colony formation, anchorage-independent growth, migration, invasion, EMT, and tumor xenograft growth, and inhibits apoptosis.
Molecular mechanism: circ_0067934 acts as a molecular sponge for miR-1301-3p, thereby increasing HMGB1 expression and activating PI3K/Akt and MAPK signaling.
Biological pathway or process:

PI3K/AKT (promotes); MAPK (promotes); proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); apoptosis (inhibits); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RNase R Treatment; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; TUNEL; IHC (Immunohistochemistry); Western Blot; In Vivo Animal Model; Bioinformatics Analysis
Clinical significance:

High expression was positively correlated with larger tumor size, advanced tumor stage, and lymph node metastasis; circ_0067934 might be a prognostic marker or therapeutic target for PTC treatment.
Description:

circ_0067934 is upregulated in papillary thyroid carcinoma tissues and cell lines and acts as an oncogenic circRNA. It promotes malignant phenotypes by sponging miR-1301-3p, increasing HMGB1 expression, and activating PI3K/Akt and MAPK signaling. High expression is associated with aggressive clinicopathological features, suggesting potential prognostic and therapeutic relevance.
Confidence score:

0.8006
Other information
Title:

circ_0067934 promotes the progression of papillary thyroid carcinoma cells through miR-1301-3p/HMGB1 axis.

Journal: Neoplasma
Published: 2022
PubMed ID: 34704451
Study type:

combined biological and clinical study
Data availability: -
Code availability: -