| Expression pattern: |
UP |
| Associated gene: |
PRKCB, PKCbeta1 |
| Associated microRNA: |
miR-197-3p |
| Biological function: |
Promotes gastric cancer development by inhibiting apoptosis and enhancing cell viability, migration and invasion; promotes tumor growth in vivo. |
| Molecular mechanism: |
Targets (sponges) miR-197-3p to relieve suppression of PRKCB, increasing PRKCB/PKCbeta1 expression and promoting GC progression. |
| Biological pathway or process: |
apoptosis (inhibits); proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; Sanger Sequencing; RNase R Treatment; RT-qPCR; Luciferase Reporter Assay; RNA Pull-Down; Transfection; Annexin V/PI Flow Cytometry; MTT; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; Western Blot; IHC (Immunohistochemistry); Clinical Sample Validation |
| Clinical significance: |
hsa_circ_0092306 expression was positively related to tumor size, histological grade, TNM stage, and lymph node metastasis. |
| Description: |
hsa_circ_0092306 is up-regulated in gastric cancer tissues and cell lines and promotes GC cell viability, migration/invasion while inhibiting apoptosis. Mechanistically, it binds miR-197-3p and upregulates PRKCB/PKCbeta1, forming a hsa_circ_0092306-miR-197-3p-PRKCB regulatory axis that drives tumor growth in vitro and in xenograft models. |
| Confidence score: |
0.8442 |