circRNA basic information
circBase ID: hsa_circ_0006404
Name: hsa_circ_FOXO3
Synonym: circ-FOXO3
Host Gene: FOXO3
Genomic location(hg19): chr6:108984657-108986092:+
Genomic location(hg38): chr6:108663454-108664889:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; healthy tissues adjacent to the tumor
Cell lines:

HT29; HCT116; HCT8; LOVO; SW480; SW620; FHC
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: LATS1
Associated microRNA: miR-543
Biological function: Suppresses colorectal cancer cell proliferation, migration and invasion; inhibits metastasis and progression
Molecular mechanism: Acts as a miR-543 sponge to increase LATS1 expression (ceRNA mechanism)
Biological pathway or process:

ceRNA regulation (other); proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Luciferase Reporter Assay; RNA Pull-Down; Northern Blot; Western Blot; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis
Clinical significance:

Downregulation of circ-FOXO3 was associated with poor overall survival of patients with CRC and associated with tumor size, distant metastasis, differentiation, lymph node metastasis and TMN stages
Description:

circ-FOXO3 (hsa_circ_0006404) is down-regulated in colorectal cancer tissues and cell lines. Functionally, circ-FOXO3 suppresses CRC cell proliferation, migration and invasion by acting as a ceRNA that sponges miR-543 and thereby increases LATS1 expression. Low circ-FOXO3 expression is associated with poor overall survival and adverse clinicopathological features.
Confidence score:

0.8244
Other information
Title:

Identification of the tumor‑suppressive role of circular RNA‑FOXO3 in colorectal cancer via regulation of miR‑543/LATS1 axis.

Journal: Oncology reports
Published: 2021
PubMed ID: 34549306
Study type:

combined biological and clinical study
Data availability: The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Code availability: -