| Expression pattern: |
UP |
| Associated gene: |
DDB1, CUL4A, p27 |
| Associated microRNA: |
- |
| Biological function: |
promotes multiple myeloma cell proliferation; inhibits apoptosis |
| Molecular mechanism: |
circRFWD2 is translated into circRFWD2_369aa and forms a CUL4A-DDB1-circRFWD2 E3 ligase complex to promote ubiquitination-mediated degradation of p27 |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); ubiquitination (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; RT-qPCR; Clinical Sample Validation; Cohort Study; Survival Analysis; Transfection; Western Blot; CCK8; Annexin V/PI Flow Cytometry; In Vivo Animal Model; Co-IP; IF (Immunofluorescence); Bioinformatics Analysis |
| Clinical significance: |
circRFWD2 was significantly more abundant in MM patients than healthy individuals; higher circRFWD2 levels were associated with inferior event-free survival |
| Description: |
circRFWD2 is up-regulated in multiple myeloma patient samples and higher levels correlate with inferior event-free survival. It can be translated into circRFWD2_369aa, which interacts with DDB1 and CUL4A to form an E3 ligase complex that promotes p27 ubiquitination/degradation, thereby enhancing MM cell proliferation and reducing apoptosis. |
| Confidence score: |
0.8347 |