| Expression pattern: |
DN |
| Associated gene: |
LARP4, Snail1, Slug |
| Associated microRNA: |
miR-223-3p |
| Biological function: |
circBNC2 acts as a tumor suppressor in EOC; overexpression inhibits cell viability, proliferation, migration, invasion, EMT-related protein expression and tumor growth, and promotes apoptosis and G0/G1 cell-cycle arrest. |
| Molecular mechanism: |
circBNC2 functions as a ceRNA sponge for miR-223-3p, thereby positively regulating LARP4 expression and suppressing EOC progression; circBNC2 overexpression also reduces EMT-related proteins Snail1 and Slug. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); cell cycle (inhibits); EMT (inhibits); metastasis (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis; Cohort Study |
| Clinical significance: |
circBNC2/miR-223-3p/LARP4 axis may be a potential regulatory mechanism for the treatment of EOC. |
| Description: |
circBNC2 is down-regulated in epithelial ovarian cancer tissues and cell lines and functions as a tumor suppressor. It sponges miR-223-3p to increase LARP4 expression, thereby inhibiting EOC cell proliferation, migration, invasion, EMT-associated protein expression, and xenograft tumor growth while promoting apoptosis. |
| Confidence score: |
0.8467 |