circRNA basic information
circBase ID: -
Name: hsa_circ_WDR25
Synonym: circWDR25 / hsa_circ_004310
Host Gene: WDR25
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm; exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissues; peritumoral liver tissues; intratumoral tissues; subcutaneous xenograft tumors
Cell lines:

SMMC-7721; Hep3B; HCCLM3; LX-2; HEK293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: ALOX15, CTLA-4, PD-L1, E-cadherin, N-cadherin, vimentin
Associated microRNA: miR-4474-3p
Biological function: Promotes HCC cell proliferation, migration, invasion, tumor growth, EMT, and immune checkpoint-related PD-L1 and CTLA-4 expression; high peritumoral expression predicts poor prognosis and recurrence.
Molecular mechanism: HSC-derived exosomal and endogenous circWDR25 acts as a ceRNA sponge for miR-4474-3p, upregulates ALOX15 expression, induces EMT, and increases PD-L1 in HCC cells and CTLA-4 in HSCs.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes); immune regulation (other); metastasis (promotes)
Detected method:
Q
H
M
Validation methods:

Microarray; RT-qPCR; ISH (In Situ Hybridization); FISH / smFISH; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High peritumoral circWDR25 expression was associated with OS and RFS, predicted poorer prognosis, and was an independent predictor for OS and RFS in patients with HCC.
Description:

This study identifies HSC-derived exosomal circWDR25 as an oncogenic circRNA in HCC. circWDR25 promotes HCC proliferation, migration, invasion, tumor growth, EMT, and immune checkpoint-related PD-L1/CTLA-4 expression mainly through the circWDR25/miR-4474-3p/ALOX15 regulatory axis. Clinically, high peritumoral circWDR25 expression is associated with poor OS and RFS and may serve as a prognostic biomarker.
Confidence score:

0.8191
Other information
Title:

Hepatic stellate cell exosome-derived circWDR25 promotes the progression of hepatocellular carcinoma via the miRNA-4474-3P-ALOX-15 and EMT axes.

Journal: Bioscience trends
Published: 2022
PubMed ID: 35934785
Study type:

combined biological and clinical study
Data availability: Table S3, http://www.biosciencetrends.com/action/getSupplementalData.php?ID=108; Table S6, http://www.biosciencetrends.com/action/getSupplementalData.php?ID=109; Tables S7 and S8, http://www.biosciencetrends.com/action/getSupplementalData.php?ID=107; http://www.biosciencetrends.com/action/getSupplementalData.php?ID=110; Figure S1/S2/S3/S4/S5, http://www.biosciencetrends.com/action/getSupplementalData.php?ID=106
Code availability: -