circRNA basic information
circBase ID: hsa_circ_0005785
Name: hsa_circ_ANAPC7
Synonym: circ_0005785
Host Gene: ANAPC7
Genomic location(hg19): chr12:110819556-110834257:-
Genomic location(hg38): chr12:110381751-110396452:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
684, 686
Disease MeSH ID:
D006528
Disease NCIt ID:
C3099
Disease ICD11 ID:
1294035808
Disease OMIM ID:
114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissues; adjacent normal tissues; tumor tissues; xenografted tumors

Cell lines:

THLE-2; Huh7; SNU-387; HUVEC

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: GSK3beta, ANAPC7
Associated microRNA: miR-640
Biological function: circ_0005785 promotes HCC cell viability, proliferation, invasion, migration, angiogenesis and tumor growth, inhibits apoptosis, and reduces paclitaxel sensitivity; circ_0005785 knockdown enhances the antitumor effects of paclitaxel in vitro and in vivo.
Molecular mechanism: circ_0005785 acts as a ceRNA sponge for miR-640 to regulate GSK3beta expression in PTX-treated HCC cells.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); angiogenesis (promotes); apoptosis (inhibits); ceRNA regulation (other); drug resistance (promotes); chemoresistance (promotes)

Detected method:
Q
Validation methods:

RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Annexin V/PI Flow Cytometry; Tube Formation Assay; Western Blot; In Vivo Animal Model; Bioinformatics Analysis

Clinical significance:

promising therapeutic target for HCC treatment and preclinical basis for paclitaxel-based chemotherapy

Description:

circ_0005785 is up-regulated in HCC tissues and cell lines and is reduced by paclitaxel treatment. Its knockdown enhances paclitaxel sensitivity by suppressing proliferation, migration, invasion, angiogenesis and xenograft tumor growth while promoting apoptosis. Mechanistically, circ_0005785 functions as a miR-640 sponge to regulate GSK3beta expression.

Confidence score:

0.7951

Other information
Title:

Paclitaxel inhibits hepatocellular carcinoma tumorigenesis by regulating the circ_0005785/miR-640/GSK3beta.

Journal: Cell biology international
Published: 2023
PubMed ID: 37269228
Study type:

combined biological and clinical study

Data availability: All data are available through the corresponding author.
Code availability: -