| Expression pattern: |
DN |
| Associated gene: |
RXRalpha, beta-catenin, cyclinD1, c-Myc |
| Associated microRNA: |
hsa-miR-27a-3p |
| Biological function: |
Inhibits CRC cell proliferation, colony formation, migration, invasion; suppresses tumor growth and liver metastasis in vivo. |
| Molecular mechanism: |
Acts as a ceRNA sponging hsa-miR-27a-3p to upregulate RXRalpha and inhibit Wnt/beta-catenin signaling (reducing beta-catenin, cyclinD1, c-Myc). |
| Biological pathway or process: |
Wnt/beta-catenin (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; Sanger Sequencing; divergent primers PCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Luciferase Reporter Assay; RNA Pull-Down; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Bioinformatics Analysis |
| Clinical significance: |
hsa_circ_0004194 expression was correlated with T stage and N stage in CRC patients; suggested as a therapeutic biomarker. |
| Description: |
hsa_circ_0004194 is down-regulated in colorectal cancer and acts as a tumor suppressor. It sponges hsa-miR-27a-3p to increase RXRalpha and suppress Wnt/beta-catenin signaling, thereby inhibiting CRC cell proliferation, migration/invasion and reducing tumor growth and liver metastasis in vivo. |
| Confidence score: |
0.8634 |