circRNA basic information
circBase ID: -
Name: hsa_circ_CDK8
Synonym: hsa_circ_101237
Host Gene: CDK8
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005233
MONDO name: non-small cell lung carcinoma
Disease details: Non-small cell lung cancer
Disease DO ID:
3908
Disease MeSH ID:
D002289
Disease NCIt ID:
C2926
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; nearby non-tumorous tissues

Cell lines:

A549; H1299; MRC-5

In vivo animal model:

-

circRNA-disease information
Expression pattern:
UP
Associated gene: MAPK1, Ago2
Associated microRNA: miR-490-3p
Biological function: promotes proliferation, migration, and invasion of NSCLC cells
Molecular mechanism: Acts as a ceRNA by sponging miR-490-3p to enhance MAPK1 expression
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes); MAPK (other)

Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Clinical Sample Validation; Cohort Study; Survival Analysis; Bioinformatics Analysis

Clinical significance:

High hsa_circ_101237 expression predicted poor survival in NSCLC; associated with lymph node metastasis, large tumor size and advantaged TNM stage

Description:

hsa_circ_101237 is up-regulated in NSCLC tissues and cell lines and mainly localizes to the cytoplasm. It promotes NSCLC cell proliferation, migration and invasion by acting as a ceRNA that sponges miR-490-3p, thereby increasing MAPK1 expression, and high expression is associated with poorer patient survival.

Confidence score:

0.8323

Other information
Title:

CircRNA_101237 promotes NSCLC progression via the miRNA-490-3p/MAPK1 axis.

Journal: Scientific reports
Published: 2020
PubMed ID: 32494004
Study type:

combined biological and clinical study

Data availability: 10.1038/s41598-020-65920-2
Code availability: -