| Expression pattern: |
UP |
| Associated gene: |
Cyclin D1, Bcl-2 |
| Associated microRNA: |
miR-326 |
| Biological function: |
circPUM1 promotes LUAD cell proliferation, migration, invasion and tumorigenesis, and inhibits apoptosis. |
| Molecular mechanism: |
circPUM1 acts as a miR-326 sponge and upregulates Cyclin D1 and Bcl-2 expression through a ceRNA mechanism. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (other); cell cycle (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; MTT; EdU Staining; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Luciferase Reporter Assay; Western Blot; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
circPUM1 expression was positively correlated with TNM stage and may represent a possible therapeutic target for LUAD. |
| Description: |
circPUM1 is upregulated in LUAD tissues and cell lines and acts as an oncogenic circRNA. It promotes proliferation, migration, invasion and in vivo tumorigenesis while inhibiting apoptosis, mainly by sponging miR-326 and increasing Cyclin D1 and Bcl-2 expression. Its expression is positively associated with TNM stage, suggesting potential therapeutic relevance. |
| Confidence score: |
0.6544 |