| Expression pattern: |
UP |
| Associated gene: |
HNRNPD, ATF3, METTL14 |
| Associated microRNA: |
- |
| Biological function: |
promotes proliferation, tumourigenicity, migration, invasion and metastasis in HCC |
| Molecular mechanism: |
circSTX6 binds (sponges) HNRNPD protein, facilitating HNRNPD binding to ATF3 mRNA and promoting ATF3 mRNA decay; circSTX6 expression is regulated by METTL14-mediated m6A modification. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); m6A modification (other) |
| Detected method: |
Q
H
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; ISH (In Situ Hybridization); Clinical Sample Validation; Cohort Study; Survival Analysis; ROC Analysis; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Colony Formation Assay; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; Western Blot; MeRIP / MeRIP-seq; Bioinformatics Analysis; RNA-seq |
| Clinical significance: |
upregulation of circSTX6 in HCC patients was associated with worse overall survival (OS) and recurrence-free survival (RFS); circSTX6 could distinguish between HCC tumour and normal tissues (AUC=0.8565); overexpression of circSTX6 could be considered as an independent risk factor for HCC overall survival |
| Description: |
circSTX6 (hsa_circ_0007905), derived from STX6, is up-regulated in HCC tumor tissues and predicts worse survival. It predominantly localizes to the cytoplasm where it binds HNRNPD, enhancing HNRNPD-mediated ATF3 mRNA decay, thereby promoting HCC proliferation and metastasis; its expression is modulated by METTL14-dependent m6A modification. |
| Confidence score: |
0.9026 |