circRNA basic information
circBase ID: -
Name: hsa_circ_GFRA1
Synonym: circGFRA1 / hsa_circ_005239
Host Gene: GFRA1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008315
MONDO name: prostate cancer
Disease details: prostate cancer / PCa
Disease DO ID:
 10283
Disease MeSH ID:
 D011471
Disease NCIt ID:
 C7378
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

PCa tumor tissues; adjacent normal tissues
Cell lines:

DU145; PC-3; LNCap; 22Rv1; RWPE-1
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: LMX1B, HECTD1, Ago2
Associated microRNA: miR-3064-5p
Biological function: Promotes proliferation and migration and promotes immune escape of PCa cells.
Molecular mechanism: Transcription factor LMX1B binds the GFRA1 promoter to regulate circGFRA1 expression; circGFRA1 acts as a ceRNA sponge for miR-3064-5p to upregulate HECTD1.
Biological pathway or process:

proliferation (promotes); migration (promotes); immune regulation (promotes); ceRNA regulation (promotes)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Actinomycin D / DRB Stability Assay; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Transwell Assay; ELISA; Western Blot; ChIP / ChIP-seq; Bioinformatics Analysis; Flow Cytometry（Non-apoptosis/cycle）
Clinical significance:

-
Description:

circGFRA1 is upregulated in prostate cancer tissues/cell lines and acts as an oncogenic circRNA that promotes proliferation, migration, and immune escape. Mechanistically, LMX1B activates circGFRA1 transcription, and circGFRA1 sponges miR-3064-5p to upregulate HECTD1.
Confidence score:

0.7998
Other information
Title:

LMX1B Activated Circular RNA GFRA1 Modulates the Tumorigenic Properties and Immune Escape of Prostate Cancer.

Journal: Journal of immunology research
Published: 2022
PubMed ID: 35832649
Study type:

combined biological and clinical study
Data availability: The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Code availability: -