| Expression pattern: |
UP |
| Associated gene: |
FoxM1, AGO2 |
| Associated microRNA: |
miR-548b |
| Biological function: |
Promotes GBM/glioma progression, proliferation, invasion, and in vivo tumor growth; circBFAR knockdown inhibits proliferation, invasion, and glioma growth and prolongs survival in xenograft mice. |
| Molecular mechanism: |
circBFAR acts as a cytoplasmic ceRNA that sponges miR-548b, relieving miR-548b-mediated suppression of FoxM1 and thereby promoting glioma cell proliferation and invasion. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); ceRNA regulation (promotes); metastasis (promotes); other pathway/process (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Western Blot; Nuclear-Cytoplasmic Fractionation; In Vivo Animal Model; H&E Staining; Survival Analysis; Cohort Study; Bioinformatics Analysis |
| Clinical significance: |
High circBFAR expression was associated with worse prognosis in GBM patients and positively correlated with patients' age and mean tumor diameter. |
| Description: |
circBFAR is an up-regulated human circRNA derived from BFAR in GBM tissues and glioma cell lines. It promotes glioma proliferation, invasion, and in vivo tumor growth by acting as a ceRNA for miR-548b and increasing FoxM1 expression. High circBFAR expression is associated with worse overall survival in GBM patients. |
| Confidence score: |
0.8655 |