| Expression pattern: |
UP |
| Associated gene: |
LASP1, Ago2 |
| Associated microRNA: |
miR-338-3p, miR-646 |
| Biological function: |
Promotes malignant progression of PTC by enhancing proliferation and invasion, sustaining spheroid formation/tumorigenic properties, inhibiting apoptosis, and promoting tumor growth in vivo. |
| Molecular mechanism: |
Acts as a cytoplasmic miRNA sponge for miR-338-3p, thereby relieving repression of LASP1 and promoting malignant phenotypes. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Actinomycin D / DRB Stability Assay; RNase R Treatment; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis; Western Blot |
| Clinical significance: |
High circTIAM1 expression is associated with lower overall survival in patients with PTC and may serve as a potential prognostic biomarker. |
| Description: |
circTIAM1 is up-regulated in papillary thyroid cancer tissues and cell lines and acts as an oncogenic circRNA. It mainly localizes in the cytoplasm and promotes proliferation/invasion and tumor growth by sponging miR-338-3p to upregulate LASP1. |
| Confidence score: |
0.8635 |