| Expression pattern: |
UP |
| Associated gene: |
PTBP1, PLOD3 |
| Associated microRNA: |
- |
| Biological function: |
promotes EMT; promotes migration and invasion; promotes glioblastoma progression |
| Molecular mechanism: |
EV-mediated transfer of circ_0003137; circ_0003137 physically binds PTBP1 to stabilize PLOD3 mRNA, promoting EMT |
| Biological pathway or process: |
EMT (promotes); migration (promotes); invasion (promotes); ceRNA regulation (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; Bioinformatics Analysis; RT-qPCR; RNase R Treatment; Transfection; RIP (RNA Immunoprecipitation); RNA Pull-Down; Actinomycin D / DRB Stability Assay; Western Blot; IHC (Immunohistochemistry); IF (Immunofluorescence); Transwell Assay; In Vivo Animal Model; Survival Analysis |
| Clinical significance: |
higher circ_0003137 was related to poorer survival of glioblastoma patients |
| Description: |
In glioblastoma, hypoxia-induced M2-like TAMs deliver extracellular-vesicle-enriched circ_0003137 to tumor cells, increasing its level. circ_0003137 promotes EMT, migration and invasion by binding PTBP1 and enhancing PLOD3 mRNA stability, and its knockdown suppresses tumor progression and improves survival in an intracranial xenograft model. |
| Confidence score: |
0.8432 |