| Expression pattern: |
UP |
| Associated gene: |
HMGA2 |
| Associated microRNA: |
miR-216b |
| Biological function: |
Promotes 5-FU resistance, migration and invasion, and inhibits apoptosis in breast cancer cells; promotes tumor growth in vivo. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-216b to regulate HMGA2 (ceRNA axis), thereby promoting 5-FU resistance. |
| Biological pathway or process: |
chemoresistance (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes); proliferation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Transfection; CCK8; Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Luciferase Reporter Assay; RNA Pull-Down; Western Blot; In Vivo Animal Model; Clinical Sample Validation |
| Clinical significance: |
High expression of circFBXL5 was associated with tumor size and TNM stage in breast cancer patients. |
| Description: |
circFBXL5 is up-regulated in breast cancer, especially in 5-FU-resistant cells, and promotes 5-FU resistance, migration/invasion, and tumor growth while inhibiting apoptosis. Mechanistically, it sponges miR-216b to de-repress HMGA2, forming a circFBXL5/miR-216b/HMGA2 axis. |
| Confidence score: |
0.7416 |