| Expression pattern: |
UP |
| Associated gene: |
cyclin D |
| Associated microRNA: |
- |
| Biological function: |
promotes proliferation and migration; blocks cell cycle progression when silenced |
| Molecular mechanism: |
Mechanism not experimentally determined; authors suggest possible post-transcriptional regulation (e.g., miRNA binding; complexes with cell cycle proteins). |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); cell cycle (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RT-qPCR; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; Clinical Sample Validation |
| Clinical significance: |
High expression level associated with poor differentiation, I-II T stage, and I-II TNM stage; potential biomarker and therapeutic target of ESCC. |
| Description: |
hsa_circ_0067934 is up-regulated in ESCC tumor tissues and ESCC cell lines. Its knockdown suppresses proliferation, migration and invasion, and causes G2-phase cell-cycle arrest, suggesting an oncogenic role in ESCC and potential as a biomarker/therapeutic target. |
| Confidence score: |
0.675 |