| Expression pattern: |
UP |
| Associated gene: |
Runx2, Ago2 |
| Associated microRNA: |
miR-1251-5p |
| Biological function: |
promotes VSMC calcification; promotes arterial calcification |
| Molecular mechanism: |
ceRNA sponging of miR-1251-5p to upregulate Runx2; direct binding to Runx2 protein to increase Runx2 stability/expression |
| Biological pathway or process: |
ceRNA regulation (promotes); calcification (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
Elevated levels of plasma EVs circ_0008362 were associated with more severe coronary and aorta artery calcification in patients with DM. |
| Description: |
hsa_circ_0008362 is enriched in endothelial cell-derived extracellular vesicles under high-glucose/diabetic conditions and is transferred to VSMCs to promote vascular/arterial calcification. It promotes calcification by sponging miR-1251-5p to increase Runx2 and also by directly binding Runx2 protein to enhance its stability. Circulating plasma EV hsa_circ_0008362 is elevated in DM patients and correlates with coronary/aortic calcification severity. |
| Confidence score: |
0.8499 |