| Expression pattern: |
UP |
| Associated gene: |
SOX4 |
| Associated microRNA: |
miR-138-5p |
| Biological function: |
promotes proliferation, migration, invasion, and EMT in hepatocellular carcinoma cells |
| Molecular mechanism: |
Acts as a ceRNA by binding (sponging) miR-138-5p to upregulate its target gene SOX4, thereby promoting malignant behaviors and EMT. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Transfection; MTT; Transwell Assay; Western Blot; Luciferase Reporter Assay; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
High intratumoral circ-TLK1 expression was closely associated with large tumor size, advanced TNM stage, and vascular invasion of HCC patients. |
| Description: |
circ-TLK1 is up-regulated in hepatocellular carcinoma tissues and cell lines and mainly localizes to the cytoplasm. It promotes HCC proliferation, migration, invasion, and EMT by sponging miR-138-5p and thereby de-repressing SOX4. |
| Confidence score: |
0.7372 |